Walmart, which the family owns, dropped 11% in New York trading after the Bentonville, Arkansas-based firm posted earnings that fell short of analyst expectations.
Inflationary pressures put strain on the retailer's profit margins in the first quarter, putting the company's willingness to hike prices to the test.
Sam Walton, the family's late patriarch, built the company on a discount mentality that has helped the company's shares rise during recessions in the past.
According to the Bloomberg Billionaires Index, Walton's three surviving children, Alice, Jim, and Rob, as well as daughter-in-law Christy .
Christy's son, Lukas, control just under half of the retailer, giving them a combined net worth of roughly $212 billion.
In recent years, the family, which controls its stake through several trusts, has increased its stock sales.